Anti-GRP94; clone 3C4

Application

Western Blotting Analysis: 0.5 µg/mL from a representative lot detected GRP94 in 10 µg of mouse liver and human heart tissue lysate.
Immunofluorescence Analysis: Representative lots detected GRP94 immunoreactivity in rabbit and goat ventricular myocardium sections (Vitadello, M., et al. (1998). Biochem J. 332 ( Pt 2):351-359; Vitadello, M., et al. (2001). Circulation. 103(17):2201-2206)
Immunohistochemistry Analysis: A representative lot detected GRP94 immunoreactivity in fibrillating goat atria (Vitadello, M., et al. (2001). Circulation. 103(17):2201-2206)
Western Blotting Analysis: Representative lots detected GRP94 in atrial myocytes from vairous species, including murine (Milano, G., et al. (2013). PLoS One. 8(10):e76659), goat and human (Vitadello, M., et al. (2001). Circulation. 103(17):2201-2206), and rabbit (Vitadello, M., et al. (1998). Biochem J. 332 ( Pt 2):351-359).
Immunoprecipitation Analysis: A representative lot co-immunoprecipitated nNOS with GRP94 from chemically cross-linked C2C12 murine myoblasts in either proliferating or differentiating cultures (Vitadello, M., et al. (2014). Antioxid Redox Signal. 20(16):2479-2476).
Immunocytochemistry Analysis: A representative lot detected GRP94 immunoreactivity in murine C2C12 skeletal myoblasts as well as primary skeletal myocytes from new born mice (Gorza, L., and Vitadello, M. (2000). FASEB J. 14(3):461-475).

Research CategoryCell Structure

Anti-GRP94 Antibody, clone 3C4 is an antibody against GRP94 for use in Western Blotting, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Immunocytochemistry.

Research Sub CategoryAdhesion (CAMs)

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Endoplasmin (UniProt P14625; also known as 94 kDa glucose-regulated protein, Endothelial cell (HBMEC) glycoprotein, Epididymis luminal protein 35, Epididymis secretory sperm binding protein Li 125m, gp96 homolog, GRP-94, Heat shock protein 90 kDa beta member 1, Stress-inducible tumor rejection antigen gp96, Tumor rejection antigen 1) is encoded by the HSP90B1 (also known as ECGP, GP96, GRP94, HEL35; HEL-S-125m, TRA1) gene (Gene ID 7184) in human. The endoplasmic reticulum (ER) chaperone Grp94 mediates the cell surface export of molecules involved in the native immune response, mesoderm induction, muscle development, cytoprotection against oxidative stress, and calcium homeostasis. Src family tyrosine kinase Fyn-catalyzed Grp94 phosphorylation is reported to be essential for Grp94 export from ER to the Golgi apparatus in the early phase of myoblast differentiation.

Immunogen

Epitope: N-terminal half

His-tagged recombinant protein corresponding to the N-terminal of rabbit GRP94.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Protein G Purified

Format: Purified

Quality

Evaluated by Western Blotting in murine myoblast C2C12 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected GRP94 in 10 µg of murine myoblast C2C12 cell lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~94/110 kDa observed